Coding

Part:BBa_K5487100:Design

Designed by: wenzhuo yang   Group: iGEM24_UESTC-China   (2024-09-02)


TA2 M2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The vector in which it participates in the construction should include sfGFP,The main reason for co-expressing sfGFP with the anchor peptide is that the anchor peptides have a small molecular weight, making it difficult to verify their expression using conventional methods such as protein concentration assays.

Source

The anchor peptide sequence was obtained from the supplementary information in the cited literature

References

Zhao, J.; Ruan, Y.; Zheng, Z.; Li, Y.; Sohail, M.; Hu, F.; Ling, J.; Zhang, L., Gold nanoparticles-anchored peptides enable precise colorimetric estimation of microplastics. iScience 2023, 26 (6), 106823.